RotaChrom’s team has developed a revolutionary downstream preparative instrument called Industrial Scale Centrifugal Partition Chromatography (iCPC). This novel cGMP compliant system does not require nor utilize any solid stationary phase, like silica gel to achieve precision mixture separation and has been proven to be superior to conventional liquid chromatographic techniques in terms of yield and purity. In addition, the iCPC platform has radically reduced the costs and number of steps associated with downstream method development for eclectic purification challenges
(see publications section for details).
Within this system, separation occurs between two immiscible liquid mobile and stationary phases. The extraction cells, attached to a large rotor are filled with the liquid stationary phase and then immobilized by a strong centrifugal force. The mobile phase moves through the stationary phase in the form of tiny droplets. The large surface area of the droplets assures optimal interaction between the phases.
Separation of the injected sample is based on the sample component’s varying partition coefficients between the mobile and stationary phases. The partition coefficient dictates the amount of time each molecule spends within the mobile and stationary phases and therefore the rate at which each molecule travels through the system. At the end of the loop, automated fraction collectors separate and collect all selected fractions.
RotaChrom designed its top performing extraction cells through experimentation on Computed Fluid Dynamic simulation software. After thousands of simulations, this tool revealed the drawbacks of conventional CPC cell designs and enabled RotaChrom to create unique extraction cell designs for unique purification challenges.
Drawbacks of Conventional Cell Designs as Revealed by Simulations and Flow Visualizations:
- Circular motion of liquid inside of conventional cells causes back mixing
- Scale-up of the cells was problematic. Flow dynamics drastically changed during scale-up
- Plate-number and stationary phase retention suffered due to bad flow dynamics
Through trial and error, RotaChrom’s team has designed the most elegant extraction cell solution in the industry. Our cell design combines experience from HPLC technology with the capabilities of CPC.
Advantages of RotaChrom’s Cell Design:
- Injection plug: The injection plug is responsible for the even injection of mobile phase into the stationary phase. Rotachrom’s Platform efficiently eliminates peak-broading effects for large cells and increases plate number by 2 or 3 fold for flow rates over 1l/min
- Tubular cell wall with filling: The circular cross-section of the cell not only enables predictable flows for different flow rates, but also guarantees easy manufacture, maintenance and cleaning of the cells. The possibility to use swab-probing eliminates issues with CPC cleaning validation and GMP regulatory compliance. The filling guarantees even flows of the droplets and increases the plate number by at least 50%
- Collection plug: Our design increases the solvent retention, which increases loading capacity and chromatographic resolution by 20% for the same column size.
Finally, one of RotaChrom’s most impactful discoveries was that our extraction cells can be scaled from 10 mm to 100 mm without impacting performance and increasing flows from mL/mins to multiple L/mins. Furthermore, our unique rotor design allows for these cell sizes to be interchangeable on the same machine (see scale up tab for more details).
Industrial Scale Centrifugal Partition Chromatography is proven to be superior to conventional preparative chromatographic techniques in terms of precision performance, Adaptability, Scale up and cost effectiveness. (see Advantages for more details)