Centrifugal partition chromatography (CPC) is a method belonging to the countercurrent separation techniques wherein the stationary and mobile phases used in the separation are both liquids. The principle to retain the stationary phase inside the rotor by means of centrifugal force (thus making the phase suitable as stationary phase).
The operating principle of CPC (the driving force of the separation) lies in that different components of the mixture (sample) have a different partition coefficient (K) in the separated phases (K = concentration in the upper phase divided by the concentration in the lower phase). Due to the centrifugal force arising from the rotation around the central axis, the liquid chosen to be stationary phase retained in the extraction cells remains stationary. The liquid chosen to be the mobile phase is pumped through these extraction cells. As a result of the mixing and settling operations, extraction occurs in each of these extraction cells and ducts.