• RotaChrom CPC Instruments & Solutions
  • Technology
  • About Us
  • FAQ
  • Blog
  • Webinars
  • Podcasts
  • Pressroom
  • Contact
Here you can see the logo of RotaChrom Technologies.
Here you can see the logo of RotaChrom Technologies.
  • Products
    • CPC Modeler
    • GMP rCPC
    • rCPC
    • iCPC
    • Continuous CPC
    • pCPC
    • SKIDS
  • Pricing
  • Technology
  • Applications
    • Pharma and biotech
    • Cosmetics and Fragrances
    • Food and Beverages
    • Botanical applications
  • Calculator
  • Company
    • About Us
    • Events
  • Resources
    • Blog
    • Webinars
    • Publications
    • Pressroom
    • FAQ
    • Podcasts
  • Contact
    • Career
    • Contact
    • Book a meeting

The Secret to Better Analysis: Centrifugal Partition Chromatography

Header to SelectScience interview post
February 2, 2023
In Education, Interviews, News
Share via Share via Share via Share via

Centrifugal partition chromatography is a powerful yet underutilized separation technique that can save costs and increase scalability in your lab. Despite the popularity of HPLC-based methods, these techniques may not be as efficient when scaling up or out for commercial production. Centrifugal partition chromatography offers a resource-efficient and easy-to-use alternative that can help you reach your objectives. This technique is often referred to as the “underdog” of separation methods, but it deserves a place in your lab.

Don’t Overlook CPC, The Reliable Separation Method for Your Lab

“CPC is a relatively old technology. However, it remains under-used among separation scientists,” says Dr. András Gáspár, Chief Product Officer at RotaChrom. “We are working on creating awareness for this technology because we truly believe it has the potential to complement traditional separation techniques.”

Centrifugal partition chromatography (CPC) is a liquid-liquid chromatography method that uses the principles of partitioning to separate analytes based on their differing partition coefficients in the two-liquid system. The stationary liquid phase is retained in the rotor by centrifugal forces, while the mobile phase containing the analytes is fed under pressure into the stationary phase.

Advantages of CPC and Liquid-Liquid Chromatography

CPC is commonly used as a pre-treatment step to purify or prepare samples before they are further analyzed using traditional chromatography methods, which provide higher resolution of separation. This approach allows for processing of bulk samples in a faster and more cost-effective manner, as CPC is less expensive and less prone to breakdowns under high-throughput pressure, as compared to high-cost HPLC platforms.

One of the main advantages of CPC is its liquid-liquid nature, providing a large stationary phase ratio compared to other solid-support technologies. This aspect gives CPC a high “holdability” of analytes, high capacity for separation, and robustness of operation, as explained by Dr. Árpád Könczöl, Lead Scientific Officer at RotaChrom.

“Additionally, the mechanism of separation is really simple,” Könczöl continues. “It’s based on the partition behavior of the compounds in the mixture. So, we’re able to use the retention volume equation to precisely predict the chromatogram even before separation. This predictability of CPC makes scaling or transferring the method much easier.”

Flexibility and a Wide-Range of Applications

CPC offers a high level of solvent flexibility, which can help reduce operational costs in industrial applications. “CPC allows for the use of a wide range of solvents and offers a greater chemical space to adjust solvent selectivity compared to other chromatography technologies,” said Gáspár. “This kind of flexibility is not commonly found in column chromatography and can be costly in terms of consumables. Using CPC for downstream separation can be a more cost-effective solution, especially when a team is focused on a high-value target.”

CPC has a wide range of applications, including the separation of small-molecule drug compounds, natural products, medicinal chemistry workflows, fermentation-based biotechs, and extracts for the food and beverage sector, among others in the pharmaceutical industry.

RotaChrom has innovated in the field of preparative purification with their Industrial-Scale Centrifugal Partition Chromatography method. This technology diverges from traditional methods that utilize a solid stationary phase, instead adopting a unique approach for precise molecular separation. The outcome is a system that boasts higher yield and purity, lower costs, and streamlined downstream method development for a wide array of purification needs.

Our solution can be applied in numerous industries, including pharmaceuticals, food and beverage, natural extracts, cosmetics, and fragrances. The CPC system offered by RotaChrom is highly versatile, capable of handling everything from small molecule APIs to macromolecules, and from hydrophobic lipids to completely hydrophilic peptides, all on the same instrument.

To learn more about how you can put CPC to use in your purification process, visit RotaChrom’s application library for real-life examples.

Previous article
Unlocking the Power of Centrifugal Partition Chromatography
Next article
Chromatography + Chirality = The Perfect Match: Unlocking Their Potential

Related News

Other posts that you should not miss.
CPhI Worldwide in Madrid - RotaChrom booth

RotaChrom at CPhI Worldwide

October 25, 2018
-
Exhibitions, News
CPhI Worldwide together with co-located events ICSE, InnoPack, P-MEC and FDF, hosted more than 45,000 visiting pharma professionals over three days in October 2018.
Read More →
1 MIN READ
RotaChrom exhibition - CPhI_Shanghai_3

Exhibiting at CPhI China

June 22, 2019
-
Exhibitions, News
Exhibiting at CPhI & P-MEC China, RotaChrom enters Chinese pharmaceutical market.
Read More →
1 MIN READ

Recovery, Throughput and Purity – The Chromatographer’s Triangle

January 18, 2023
-
Education, News
Chromatographic methods are used to separate and purify individual components of a mixture. Purifying compounds, however easily become a balancing act. Throughput, recovery and purity are 3 essential…
Read More →
5 MIN READ

Latest News

Advancing Separation Science: CPC Device Installation and Its Impact

April 17, 2025 – News

RotaChrom at Flow Chemistry Europe 2025: Exploring Innovation Through CPC Technology

April 16, 2025 – Events, News

SZTE’s Award-Winning Innovation: A Natural Breakthrough in Obesity Prevention

February 4, 2025 – Education, News, Studies

Innovations in Chiral Purification: Exploring Techniques and Future Potential

January 31, 2025 – Download, Education, News

Webinars

Separation of Remdesivir Diastereomeres by Centrifugal Partition Chromatography

September 25, 2023

Green Features of Centrifugal Partition Chromatography

May 10, 2021

Contact Us

RotaChrom Technologies PLC (Headquarters)

Csillag street 2/A, Kecskemet, HU-6000

+36 70 885 6922

RotaChrom North America Inc.

300 Spectrum Center Drive, Irvine, CA 92618

+1 (720) 220-5669

Cookie Policy  /  Data Protection Policy  /  RotaChrom® is a registered trademark of Rotachrom Technologies in the USA and EU. / © 2025 RotaChrom Technologies. All rights reserved.
CPC: The Secret to Better Analysis and Separation | RotaChrom