BOTANICALS
Botanicals &
Plant-Derived Compounds
Centrifugal Partition Chromatography (CPC) provides a highly selective, scalable, and environmentally responsible solution for the purification of complex botanical extracts and plant-derived compounds.
Its liquid–liquid separation principle eliminates the need for costly solid supports, avoiding irreversible adsorption and minimizing sample loss—critical when working with rare or high-value natural products. CPC’s gentle, low-shear operating conditions preserve the structural integrity and bioactivity of sensitive molecules.
With excellent scalability from laboratory to industrial production. Its high selectivity improves target compound recovery while enabling remediation of undesired components to meet strict regulatory thresholds. In addition, CPC supports green manufacturing principles through reduced solvent consumption, full solvent recyclability, and compatibility with quality management systems and industry standards in the botanical and nutraceutical sectors. This combination of performance, sustainability, and compliance makes CPC a preferred choice for modern plant-based ingredient production.
Purification of CANNABINOIDS
Centrifugal Partition Chromatography (CPC) plays a key role in cannabinoid separation, enabling efficient purification of compounds such as CBD, CBN, and other minors, while eliminating THC.
Unlike traditional chromatography, CPC uses liquid–liquid partitioning without solid supports, eliminating costly stationary phases and reducing material loss.
It achieves pharmaceutical-grade purity with high recovery, scalable throughput, and complete regulatory compliance, making it ideal for producing safe, consistent, and high-value cannabinoid ingredients.
Cannabinoids
+ Tetrahydrocannabinol (THC) removal from CBD crude
THC Removal
Tetrahydrocannabinol (THC), a psychoactive cannabinoid present in hemp-derived CBD crude extract, must be removed to meet regulatory standards. Using an industrial-scale pCPC system, THC was eliminated, yielding CBD with 99% purity and no detectable THC.
The method delivered 88% recovery, minimizing product loss while remaining cost-effective. Scalable to 8.2 kg per 8-hour shift, it ensures high-purity CBD for natural extracts, nutraceutical, and cosmetics industries.
+ Tetrahydrocannabinol (THC) removal from CBD mother liquor
THC Removal
Cannabidiol (CBD), obtained from crystallization mother liquor, requires removal of residual THC to meet compliance standards. Using an rCPC system, THC was fully eliminated in just 35 minutes per run with 95 g injections.
The purified fraction met strict regulations while enriching minor cannabinoids like CBN and CBG, unlocking added therapeutic value. With its speed, efficiency, and scalability, rCPC ensures both compliance and recovery of high-value compounds for diverse industries.
+ Tetrahydrocannabinol (THC) removal from CBD distillate
THC Removal
Tetrahydrocannabinol (THC), a psychoactive cannabinoid present in hemp-derived CBD distillate, must be removed to meet strict regulations. Using RotaChrom’s rCPC system, a distillate containing 73% CBD and 9% THC was purified to 97% CBD with THC reduced to undetectable levels, achieving 95% recovery.
Scalable to industrial volumes, the process purified 1.858 kg in an 8-hour shift, ensuring compliance, efficiency, and cost-effectiveness.
+ Cannabidiol (CBD) Isolation from Distillate
CBD Isolation
Cannabidiol (CBD), obtained from CBD distillate, requires removal of THC to reach pharmaceutical quality. Using an rCPC system, CBD was purified to >99.5% with complete THC elimination, fully compliant with clinical-grade standards.
The process ensures efficiency, scalability, and cost-effectiveness, delivering THC-free CBD suitable for pharmaceutical, nutraceutical, and cosmetic applications, where purity, safety, and regulatory compliance are essential.
+ Tetrahydrocannabinol (THC) removal from a distillate
THC Isolation
THC (Δ⁹-tetrahydrocannabinol), a clinically important cannabinoid with applications in pain relief, appetite stimulation, and nausea reduction, can be efficiently purified from CBD distillate using RotaChrom’s rCPC system. Our method achieves over 99% purity with an 85% recovery rate, demonstrating pharmaceutical-grade performance.
This scalable, cost-effective approach provides consistent, high-quality THC suitable for use in regulated medical and research applications, supporting the development of cannabinoid-based therapeutics.
+ Cannabinol (CBN) isolation from a distillate
CBN Isolation
Cannabinol (CBN), a rare cannabinoid known for its sleep-support, relaxation, and anti-inflammatory benefits, was purified from distillate using an rCPC system. The process achieved >99.5% purity with a 95% yield, while efficiently removing THC and minimizing CBD content.
In just 34 minutes, pharmaceutical-grade CBN was obtained, making rCPC a fast, scalable, and cost-effective solution for producing premium, regulatory-compliant CBN extracts.
REMEDIATION of aLKALOIDS
Centrifugal Partition Chromatography (CPC) is a powerful tool for removing toxic alkaloids from plant extracts while preserving valuable active compounds such as isoflavones and cannabinoids.
By leveraging liquid–liquid separation, CPC achieves regulatory-compliant alkaloid levels with high recovery and no risk of stationary phase contamination.
Scalable and solvent-efficient, CPC ensures safe, high-quality extracts suitable for nutraceutical, pharmaceutical, and cosmetic applications.
Alkaloids
+ Pyrrolizidine Alkaloid
Pyrrolizidine Alkaloid Remediation from Red Clover
Pyrrolizidine alkaloids (PAs), toxic compounds with hepatotoxic and carcinogenic risks, were removed from Red Clover (Trifolium pratense) extracts while preserving valuable isoflavones. Using rCPC in continuous mode, PA levels were reduced below 400 ppb without loss of therapeutic compounds.
Dual-mode operation tripled productivity versus batch runs, and scale-up enables up to 6.4 kg/day, providing a safe, efficient, and high-quality Red Clover extract.
CURCUMIN
+ Curcumin
High-Purity Curcuminoid Purification
The primary curcuminoids in turmeric extract—curcumin, demethoxycurcumin, and bisdemethoxycurcumin—require high-purity isolation to meet nutraceutical and pharmaceutical standards. Using an industrial-scale Centrifugal Partition Chromatography (CPC) system, non-active matrix components were efficiently removed, yielding a total curcuminoid fraction with 92.6% purity and over 90% recovery.
The scalable CPC method enables predictable transfer from pilot to industrial production, processing over 6 kg of crude extract per day while maintaining excellent selectivity and low solvent consumption. This solution delivers cost-effective, high-purity curcuminoids for nutraceutical, food additive, and advanced pharmaceutical applications.
