Here’s Why You Should Use Chromatography In Your Purification

Centrifugal Partition Chromatography (CPC) represents a revolutionary chromatographic technique that integrates traditional liquid-liquid chromatography (LLC) with the force of centrifugation. Unlike conventional chromatography, CPC employs interconnected cells in lieu of columns, leading to a robust system. The liquid stationary phase within these cells, driven by centrifugal force, facilitates efficient separation when interacting with the mobile phase.

CPC offers advantages over traditional methods, showcasing heightened selectivity and efficiency in complex mixture untangling. Its absence of solid stationary phases mitigates the risk of irreversible absorption, allowing precise molecular separation. This distinctive approach finds applications in purification, isolation, and remediation processes across various industries.

Isolation, Separation, and Remediation in Chromatography

• Isolation: Isolation involves extracting a specific Compound of Interest (CoI) from a complex matrix. This intricate task requires resolution power and the ability to selectively fractionate the CoI. Successful isolation demands thorough research and method fine-tuning to achieve optimal outcomes, especially when dealing with compounds of diverse physicochemical properties.
• Separation: In the realm of separation, the goal is to simplify the input matrix while focusing on at least two CoIs. Challenges arise when CoIs share similar chromatographic characteristics, particularly with isomers. Overcoming these challenges is crucial to achieving desired resolution between CoIs.
• Remediation: Remediation aims to remove unwanted components from the sample or matrix, ensuring the efficiency of the CoI and minimizing risks to the environment or human health. This process targets hazardous, carcinogenic, environmentally polluting, or legally restricted impurities. By eliminating detrimental components, remediation ensures a refined and safe end product.

In summary, isolation, separation, and remediation are interlinked yet distinct endeavors within chromatography, collectively guiding the path toward a purified and refined outcome.

Further Resources

To learn more about natural extracts, CPC and chromatography in general, click any of the links below.

• CPC Technology
• CPC Application Notes
• Chromatography Articles and Research