Liquid-liquid chromatography (LLC) is a technique in which the separation of mixture components is achieved due to their different distribution between the two phases of a pre-equilibrated biphasic solvent system. In this work, the LLC operation in the nonlinear range of the distribution isotherm was systematically examined for the first time.
The pharmaceutical industry is increasingly prioritizing green and sustainable practices, with a strong focus on reducing waste generated during downstream processing. Since separation technologies contribute significantly to the environmental footprint of pharmaceutical manufacturing, implementing eco-friendly alternatives is crucial.
Over the past two decades, the demand for sustainable and eco-friendly alternatives to conventional chromatographic separation techniques has led to significant advancements in greener methodologies, such as supercritical fluid chromatography (SFC), ion exchange resins, and reverse-phase high- or medium-pressure liquid chromatography (HPLC/MPLC). Among these, liquid–liquid chromatography (LLC) has emerged as a viable alternative, with centrifugal partition chromatography (CPC) standing out as a promising support-free liquid–liquid technique suitable for both pilot and industrial-scale applications.
This study comprehensively characterizes the interactions between mitragynine (MTR) and cyclodextrins (CyDs) using NMR and circular dichroism (CD) spectroscopy.
Despite ongoing research efforts, an entirely effective treatment for COVID-19 remains elusive. Interest in cannabidiol (CBD) and essential oils (EOs) as potential therapeutic agents is increasing; however, no scientific studies have yet evaluated the role of CBD and specific terpenes in the progression of SARS-CoV-2 infection. Our research focuses on inhibiting both viral entry and replication through biobased formulations derived from cannabis, thyme, and oregano.
Arpad Konczol, Mario Nacsa, Eszter Blanar Petervarine, Erika Tamas Jantyikne, Dora Rutterschmid, Laszlo Ovari, Kristof Gazda, Gergo Dargo, 2021, ‘Comprehensive review on the versatile applications of centrifugal partition chromatography in Cannabis and Hemp processing’, American Oil Chemists’ Society, Conference Poster
David Laczko, Eszter Blanar Petervarine, Arpad Konczol, Attila Hunyadi, ‘Ekdiszteroidok elválasztása méretnövelhető centrifugális megoszlásos kromatográfiával’, 2021, METT25, Conference poster
Arpad Konczol, Dora Rutterschmid, Gergo Dargo, Kristof Gazda, Laszlo Ovari, Mario Nacsa, David Laczko, Andras Gaspar, 2021, ‘Research & Development of Centrifugal Partition Chromatography’, METT25, Conference presentation
Gergo Dargo, David Laczko, Laszlo Ovari, Kristof Gazda, Mario Nacsa, Erika Jantyikne Tamas, Eszter Petervarine Blanar, Dora Rutterschmid, Laszlo Lorantfy, Arpad Konczol, 2021, ‘Centrifugal Partition Chromatography at Industrial Scale: Concept, Instrumentation and Applications’, Preparative and Process Chromatography, Conference presentation
In this study, centrifugal partition chromatography (CPC), an advanced separation technology increasingly applied in pharmaceutical and natural product purification, now reaching industrial-scale implementation, was used. As both stationary and mobile phases in CPC are liquids, solvent consumption can be substantial, necessitating automation for efficient solvent preparation and recycling. To address this challenge, we present a practical solution encompassing the concept, algorithm, and instrumentation for optimized solvent system management.
This presentation provides an overview of recent advancements and applications in centrifugal partition chromatography (CPC) and introduces a fully automated, industrial-scale CPC platform. This innovative system integrates biphasic liquid system preparation and solvent recycling units, specifically designed for the efficient separation of natural products such as cannabinoids.
Robert Orkenyi, Laszlo Ovari, Kristof Gazda, Erika Tamas, Mario Nacsa, Eszter Blanar, Dora Rutterschmid, Laszlo Lorantfy, Andrea Boszormenyi, Balazs Kramos, Arpad Konczol, 2020, ‘Cannabidiol Isolation Including Cannabis-Relevant PesticidesRemoval by Centrifugal Partition Chromatography’, American Chemical Society, Conference presentation
Arpad Konczol, Dora Rutterschmid, Robert Orkenyi, Laszlo Lorantfy, 2019, ‘Pesticide Classification System in the Isolation of Cannabidiol using Centrifugal Partition Chromatography’, Preparative and Process Chromatography, Conference poster
In this study, RotaChrom researchers employed centrifugal partition chromatography (CPC) to purify 10-DAB III, optimizing solvent systems and determining partition coefficients of taxol components using high-performance liquid chromatography (HPLC).