RotaChrom’s technology is built around a process-engineering-driven approach to chromatographic purification, where separation performance, system design, and scalability are developed as a single, integrated framework. From laboratory-scale method development to full system configuration, purification is treated as a controlled, model-based process rather than a standalone instrument operation (Figure 1).

The technology combines chromatographic method optimization with engineered solvent handling, peripheral sizing, and process simulation to ensure predictable performance at scale. This integrated design philosophy enables robust, scalable purification workflows that align separation chemistry with industrial process requirements.

Based on the client’s predefined requirements, chemists develop the most appropriate chromatography method for extracting the desired quality and quantity of material from the substance to be purified. During this phase, solvent tests are used to select the appropriate solvent system, the injectable sample volume, the retention time of the component to be purified, and the operational parameters of the CPC, such as flow rate and rotation speed.

The developed method is first tested on a laboratory-scale device. After successful method development, the operational parameters of the CPC and the required peripherals are determined in collaboration with the client. A preliminary Process Flow Diagram (PFD) is then created (Figure 2), including appropriately sized CPC equipment, sample injection skids, solvent handling skids, fraction collectors, mixers, pumps, and all required peripherals.

The PFD illustrates the sequence of connections between the CPC and all system components, as well as the flow paths through the system. Solvent recycling, combined with appropriately sized external peripherals and optimized operating parameters, ensures continuous and cost-effective operation.

The method and the necessary technological setup are designed to be flexible, allowing for future scalability and functional expansion. Additional CPC units and support peripherals can be added without compromising the operational integrity of the existing system.

The PFD serves as the foundation for purification process simulation using RotaChrom’s proprietary Process Flow Calculator (PFC). PFC determines the required capacity of external peripherals and operating parameters needed to meet the production capacity defined by the customer.

Using iterative simulations, PFC calculates ongoing flows, identifies bottlenecks, evaluates buffer and evaporator capacities, verifies realistic operating times, and assesses power and utility requirements. If errors or limitations are detected, the PFD and system configuration are revised accordingly until an optimized solution is achieved (Figure 3).

Once the optimized operational model and final PFD are confirmed, engineers proceed with the creation of the Process and Instrumentation Diagram (P&ID). The P&ID details equipment interconnections, flow directions, safety and control systems, pressure levels, and instrumentation required for operation and maintenance.

Understanding P&IDs is essential for plant operation, troubleshooting, maintenance planning, and future system modifications. This structured approach ensures that each system is optimized for the client’s current needs while remaining adaptable to future expansion.

In parallel, a precise Total Cost of Ownership (TCO) calculation is performed to assess long-term operational efficiency and investment impact. Learn more about the TCO Calculator →